SIZE KINETICS

Real-time monitoring of transfection mix complexation

Videodrop SK offers a revolutionary approach to transfection workflow optimization.

Reagent-based transfection is the primary method for producing viral vectors (LVor AAV). However, despite its significance, this critical step often lacks real-time monitoring and quality controls.

Automated Kinetics Monitoring

• Size measurement every 15s from 80 nm to 2 μm

• Ratios media type On the same sample droplet

• Media type Over a defined time period up to 4 hours

Real-Time Visualization Transfection reagent/DNA complexes formation

• Visualize real-time kinetic curves construction

• Access to Microscopic imaging

Screening of transfection mix conditions

1. Hu, Y. et al. Size-Controlled and Shelf-Stable DNA Particles for Production of Lentiviral Vectors. Nano Lett. 21, 5697–5705 (2021).

Videodrop enables in-depth real-time kinetic studies.

Scientists can monitor monitor the impact of various conditions on the size evolution of transfection complexes over time :

• DNA concentration

• Ratios media type

• Media type

• pH levels

Studies⁽¹⁾show a clear correlation between size of the formed
transfection reagent/DNA complex and the transfection efficiency.

DEMONSTRATIONS

Two concentrations of DNA (3 plasmids : 1 and 0.25μg/106 cells) were tested and mixed with the same proportion of PEIPro.

Videodrop allows to follow in real-time the DNA/PEIPro complexation and highlight the kinetics difference between two conditions.

The Simplest Analytical Tool for Real-Time Transfection Mix Analysis

By incorporating Videodrop into their workflows, scientists can achieve a new level of control and optimize transfection efficiency, ultimately leading to faster and more cost-effective upstream processes.