SIZE KINETICS
Real-time monitoring of transfection mix complexation
Videodrop SK offers a revolutionary approach to transfection workflow optimization.
Reagent-based transfection is the primary method for producing viral vectors (LVor AAV). However, despite its significance, this critical step often lacks real-time monitoring and quality controls.
Automated Kinetics Monitoring
• Size measurement every 15s from 80 nm to 2 μm
• Ratios media type On the same sample droplet
• Media type Over a defined time period up to 4 hours
Real-Time Visualization Transfection reagent/DNA complexes formation
• Visualize real-time kinetic curves construction
• Access to Microscopic imaging
Screening of transfection mix conditions
Videodrop enables in-depth real-time kinetic studies.
Scientists can monitor monitor the impact of various conditions on the size evolution of transfection complexes over time :
• DNA concentration
• Ratios media type
• Media type
• pH levels
Studies(1) show a clear correlation between size of the formed
transfection reagent/DNA complex and the transfection efficiency.
Demonstration
Two concentrations of DNA (3 plasmids : 1 and 0.25μg/106 cells) were tested and mixed with the same proportion of PEIPro.
Videodrop allows to follow in real-time the DNA/PEIPro complexation and highlight the kinetics difference between two conditions.
The Simplest Analytical Tool for Real-Time Transfection Mix Analysis
By incorporating Videodrop into their workflows, scientists can achieve a new level of control and optimize transfection efficiency, ultimately leading to faster and more cost-effective upstream processes.
